METHODS OF ANAEROBIC CULTURE
1966; Elsevier BV; Linguagem: Inglês
10.1016/b978-1-4832-3205-8.50016-7
AutoresW.F. Harrigan, Margaret E. McCance,
Tópico(s)Enzyme Production and Characterization
ResumoThis chapter discusses the methods of anaerobic culture. To grow obligate anaerobes, cultures must be kept in an oxygen-free environment, which can be achieved by the use of a natural medium containing reducing substances. Shake cultures are test tubes or bottles containing a solid medium. The melted medium is inoculated and then allowed to solidify. The simplest medium to use is nutrient agar with the addition of 1 percent glucose. Semisolid media contain 0.02–0.3 percent agar, which is sufficient to prevent convection currents and, therefore, helps to prevent the diffusion of oxygen into the medium. By the use of sterile sealing compounds, liquid media can be kept under anaerobic conditions. The method is used in the demonstration of the presence of the obligate anaerobe Clostridium welchii in water or milk by the stormy-clot reaction, in which the medium is sterile milk rendered anaerobic and with a paraffin wax seal. Vaspar is a useful sealing compound prepared by melting together approximately equal amounts of petroleum jelly and paraffin wax.
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