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Effect of denture-coating composite on Candida albicans biofilm and surface degradation after disinfection protocol

2016; Elsevier BV; Volume: 66; Issue: 2 Linguagem: Inglês

10.1111/idj.12212

1875-595X

Matheus Jacobina Silva, Denise Gusmão de Oliveira, Oscar O. Marcillo, Karin Hermana Neppelenbroek, Vanessa Soares Lara, Vinícius Carvalho Porto,

Dental Erosion and Treatment

Introduction: Denture stomatitis is the most common pathology affecting denture wearers and its main cause is colonisation of dentures with Candida albicans. Objective: This study investigated the effectiveness of two commercial composite surface sealants (Biscover® LV and Surface Coat®) to reduce C. albicans biofilm colonisation on denture resin, as well as their surface integrity after disinfection cycles with 1% sodium hypochlorite solution. Methods: Heat-cured acrylic resin specimens were manufactured (10 mm × 10 mm × 1 mm). The specimen surfaces were mechanically polished to simulate rough or smooth denture surfaces. Four surface-treatment groups were tested: smooth surfaces [0.3 μm of mean roughness (Ra)]; rough surfaces (3 μm of Ra); rough surfaces treated with Biscover® LV; and rough surfaces treated with Surface Coat®. Specimens of each group were randomly divided to undergo immersion in distilled water or 1% sodium hypochlorite for 30 or 90 cycles each. Specimens of all groups in each immersion solution were tested using a crystal violet (CV) staining assay for biofilm quantification and by scanning electron microscopy for visual analyses of surface integrity and biofilm structure. CV assay data were analysed using one-way analysis of variance (ANOVA) followed by Tukey's multiple comparison test (P < 0.05). Results: The effectiveness and surface integrity of Biscover® LV-treated surfaces were similar to those of smooth surfaces, whereas Surface Coat®-treated surfaces presented a similar performance to rough surfaces in all solutions and cycles. Conclusion: These results suggest the possibility of clinical use of Biscover® LV for denture coating on surfaces in which mechanical polish is not indicated, such as the fitting surface.