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The Xenopus laevis mitochondrial protein mtDBP-C cooperatively folds the DNA in vitro.

1988; Springer Nature; Volume: 7; Issue: 12 Linguagem: Inglês

10.1002/j.1460-2075.1988.tb03273.x

1460-2075

Bernard Mignotte, Etienne Delain, D. Rickwood, Monique BARAT‐GUERIDE,

CRISPR and Genetic Engineering

Research Article1 December 1988free access The Xenopus laevis mitochondrial protein mtDBP-C cooperatively folds the DNA in vitro. B. Mignotte B. Mignotte Laboratoire de Biologie Générale, Université de Paris-Sud, Orsay, France. Search for more papers by this author E. Delain E. Delain Laboratoire de Biologie Générale, Université de Paris-Sud, Orsay, France. Search for more papers by this author D. Rickwood D. Rickwood Laboratoire de Biologie Générale, Université de Paris-Sud, Orsay, France. Search for more papers by this author M. Barat-Gueride M. Barat-Gueride Laboratoire de Biologie Générale, Université de Paris-Sud, Orsay, France. Search for more papers by this author B. Mignotte B. Mignotte Laboratoire de Biologie Générale, Université de Paris-Sud, Orsay, France. Search for more papers by this author E. Delain E. Delain Laboratoire de Biologie Générale, Université de Paris-Sud, Orsay, France. Search for more papers by this author D. Rickwood D. Rickwood Laboratoire de Biologie Générale, Université de Paris-Sud, Orsay, France. Search for more papers by this author M. Barat-Gueride M. Barat-Gueride Laboratoire de Biologie Générale, Université de Paris-Sud, Orsay, France. Search for more papers by this author Author Information B. Mignotte1, E. Delain1, D. Rickwood1 and M. Barat-Gueride1 1Laboratoire de Biologie Générale, Université de Paris-Sud, Orsay, France. The EMBO Journal (1988)7:3873-3879https://doi.org/10.1002/j.1460-2075.1988.tb03273.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The binding of the Xenopus laevis mitochondrial protein mtDBP-C to DNA was studied by equilibrium density banding, agarose gel electrophoresis and electron microscopy. The results obtained show that the mtDBP-C binds cooperatively to DNA irrespective of whether the DNA is supercoiled, relaxed or linear and it induces the formation of superhelical turns locally leading to the formation of a highly folded structure. It appears that this protein could be involved in the compaction of DNA in the mitochondrial nucleoid. Previous ArticleNext Article Volume 7Issue 121 December 1988In this issue RelatedDetailsLoading ...