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The use of endothelial progenitor cells combined with barrier membrane for the reconstruction of peri‐implant osseous defects: an animal experimental study

2016; Wiley; Volume: 43; Issue: 3 Linguagem: Inglês

10.1111/jcpe.12511

1600-051X

Eli E. Machtei, David M. Kim, Nadeem Y. Karimbux, Hadar Zigdon‐Giladi,

Dental Implant Techniques and Outcomes

Abstract Background The aim of this research was to study the efficacy of endothelial progenitor cells ( EPC ) with ß tri‐calcium phosphate (β‐ TCP ) and barrier in the treatment of experimental peri‐implantitis. Methods Thirty implants were placed in five American foxhounds. Following osseointegration, ligature‐induced peri‐implantitis was initiated. EPC were isolated from peripheral blood. Two months later, open flap debridement was performed and implants’ surface was decontaminated with 24% EDTA . The sites were grafted with β‐ TCP and collagen membrane (β‐ TCP group) or β‐ TCP loaded with EPC and covered with membrane ( EPC group) or left un‐grafted ( OFD group). At 3 months, animals were killed and specimens sent for histomorphometric and micro‐ CT analysis. Bone height and % BIC served as primary and secondary outcomes. Results EPC group showed shorter distance to first BIC (3.29 ± 0.69 mm) compared to 4.2 ± 0.92 mm (β‐ TCP ) and 3.82 ± 0.73 mm ( OFD ). Mean histological BIC was 2–3 folds higher in the EPC group (17.65 ± 3.3%) compared with OFD (7.55 ± 2.24%, p = 0.01) and β‐ TCP (5.68 ± 2.91%, p = 0.05). BIC greater than 25% was found only in the EPC group. The distance between the implant shoulder to 1st BIC was 2.51 ± 0.7 mm (lingual sites) compared to 3.64 ± 0.8 mm for buccal sites ( p < 0.0001). Conclusion β‐ TCP loaded with EPC and covered with a membrane‐enhanced bone formation around previously contaminated dental implants in an experimental peri‐implantitis canine model.