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First Report of Tomato Gray Leaf Spot Caused by Stemphylium lycopersici in Zhejiang Province, China

2015; American Phytopathological Society; Volume: 100; Issue: 1 Linguagem: Inglês

10.1094/pdis-05-15-0615-pdn

1943-7692

Xuepiao Sun, Lian Zhang, Jin‐Zhi Zhang, Guoquan Lu,

Plant Disease Resistance and Genetics

HomePlant DiseaseVol. 100, No. 1First Report of Tomato Gray Leaf Spot Caused by Stemphylium lycopersici in Zhejiang Province, China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Tomato Gray Leaf Spot Caused by Stemphylium lycopersici in Zhejiang Province, ChinaX.-T. Sun, L. Zhang, J.-Z. Zhang, and G. LuX.-T. Sun, L. Zhang, J.-Z. Zhang, and G. LuAffiliationsAuthors and Affiliations X.-T. Sun L. Zhang J.-Z. Zhang , Institute of Biotechnology, College of Agriculture & Biotechnology, Zhejiang University, HangZhou, 310058, China G. Lu , Department of Horticulture, College of Agriculture & Biotechnology, Zhejiang University, HangZhou, 310058, China. Published Online:4 Nov 2015https://doi.org/10.1094/PDIS-05-15-0615-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat During the 2013 to 2014 growing season, symptoms of an unknown leaf spot were observed on tomato (Solanum lycopersicum L.) in the suburbs of Hangzhou City, Zhejiang Province, China. Diseased plants exhibited dark brown or grayish spots (1.5 to 3.3 in diameter) in early stages and a rapid wilting of the foliage occurred with disease development. Subsequently, complete defoliation took place as the disease progressed. A putative fungal pathogen was isolated from leaf lesions. Five single-spore isolates were grown on potato dextrose agar (PDA) and potato carrot agar (PCA) under light/dark cycle (12/12 h) for 8 to 12 days. Conidia were oblong with a conical end at the apex, bluntly rounded at the base, constricted at 3 (or 4) transverse septa, pale to mid brown, mostly 54 to 78 × 17 to 25 μm. All five isolates were identified morphologically as Stemphylium lycopersici (Enjoji) W. Yamam (Ellis 1971; Gannibal 2012). PCR amplification of genomic DNA using the primers ITS6 and ITS4, EF1-α (translation elongation factor 1-α), and GPDH (glyceraldehyde-3-phosphate dehydrogenase) generated sequences of approximately 650 bp, 850 bp and 950 bp, respectively. Five sequences of ITS region were submitted to GenBank (Accession Nos. KR911814, KR911815, KR911816, KR911817, and KR911818). These sequences were 100% identical to S. lycopersici isolated from tobacco (JX845139) (Kurose et al. 2014). Five sequences of the portion of EF-1α gene, Accession Nos. KR911804, KR911805, KR911806, KR911807, and KR911808, showed 99% identity with S. lycopersici isolate from chickpea (AB828254) (Hong et al. 2012). Five sequences of the portion of GPD gene, Accession Nos. KR911809, KR911810, KR911811, KR911812, and KR911813, showed 99% identity with S. lycopersici isolate from Wilford Swallowwort (KC160509) (Kurose and Hoang 2014). Conidial suspensions (106 conidia/ml) of each of the five isolates were inoculated into the leaves of greenhouse-grown (25 ± 2°C) tomato seedlings grown in pasteurized potting medium. On inoculated young leaves, small flecks were incited 3 days after inoculation, with water-soaked, brownish, depressed lesions appearing after 4 to 5 days; diseased leaves began to wilt after 2 weeks. Water-inocualted controls showed no symptoms. Stemphylium lycopersici was reisolated from foliar lesions. To our knowledge, this is the first record of gray leaf spot disease caused by S. lycopersici of tomato in Zhejiang Province, China.References:Ellis, M. B. 1971. Dematiaceous Hyphomycetes. CMI, Kew, Surrey, UK. Crossref, Google ScholarGannibal, P. B. 2012. Mycotaxon 121:371. https://doi.org/10.5248/121.371 Crossref, ISI, Google ScholarHong, S. K., et al. 2012. Mycobiology 40:268. https://doi.org/10.5941/MYCO.2012.40.4.268 Crossref, Google ScholarKurose, D., et al. 2014. J. Gen. Plant Pathol. 80:147. https://doi.org/10.1007/s10327-013-0501-3 Crossref, ISI, Google ScholarThe research was funded by the Public Project of Zhejiang Province (No. 2014C32012).DetailsFiguresLiterature CitedRelated Vol. 100, No. 1 January 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 15 Jan 2016Published: 4 Nov 2015First Look: 18 Aug 2015Accepted: 4 Aug 2015 Pages: 227-227 Information© 2016 The American Phytopathological SocietyCited byStemphylium lycopersici (grey leaf spot)CABI Compendium, Vol. CABI CompendiumStemphylium lycopersici Causing Leaf Spot of Watermelon (Citrullus lanatus) in ChinaHaiyan Ben, Jianfei Huo, Chengze Song, Yurong Yao, Wei Gao, Wanli Wang, Yongjuan Hao, and Xueyan Zhang3 December 2021 | Plant Disease, Vol. 105, No. 12Varietal susceptibility overcomes climate change effects on the future trends of rice blast disease in Northern ItalyAgricultural Systems, Vol. 193First Report of Stemphylium lycopersici Causing Gray Leaf Spot on Physali (Physalis alkekengi) in ChinaHuanhuan Yang, Yue Li, Yuqing He, Yaoguang Sun, Jingbin Jiang, Tingting Zhao, He Zhang, Xiangyang Xu, and Jingfu Li18 August 2020 | Plant Disease, Vol. 104, No. 10Development of an empirical tomato crop disease model: a case study on gray leaf spot28 November 2019 | European Journal of Plant Pathology, Vol. 156, No. 2Molecular re-identification of Stemphylium lycopersici and Stemphylium solani isolates deposited in NCBI GenBank and morphological characteristics of Malaysian isolates18 September 2018 | European Journal of Plant Pathology, Vol. 153, No. 3First Report of Stemphylium lycopersici Causing Gray Leaf Spot on Potato in ChinaH. 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