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Staining of Glycoproteins/Proteoglycans on SDS-Gels

1996; Humana Press; Linguagem: Inglês

10.1007/978-1-60327-259-9_99

1949-2456

Holger Jon Møller, Jørgen Poulsen,

Advanced Proteomics Techniques and Applications

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a commonly employed technique for separation of proteins according to size. Among other applications, it is used for identification and characterization of proteins on the basis of mol-wt determinations. In this chapter, staining methods will be described that permit detection of highly glycosylated proteins on SDS gels at levels of a few nanograms.