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Eradication of Methicillin-Resistant Staphylococcus aureus and of Enterobacteriaceae Expressing Extended-Spectrum Beta-Lactamases on a Model Pig Farm

2015; American Society for Microbiology; Volume: 81; Issue: 21 Linguagem: Inglês

10.1128/aem.01713-15

1098-5336

Ricarda Maria Schmithausen, Sophia Ricarda Kellner, Sophia Veronika Schulze-Geisthoevel, Sylvia Hack, Steffen Engelhart, Isabel Bodenstein, Nahed Al-Sabti, Marion Reif, Rolf Fimmers, Barbara Körber-Irrgang, Jürgen Harlizius, Achim Hoerauf, Martin Exner, Gabriele Bierbaum, Brigitte Petersen, Isabelle Bekeredjian‐Ding,

Microbial Metabolism and Applications

Colonization of livestock with bacteria resistant to antibiotics is considered a risk for the entry of drug-resistant pathogens into the food chain. For this reason, there is a need for novel concepts to address the eradication of drug-resistant commensals on farms. In the present report, we evaluated the decontamination measures taken on a farm contaminated with methicillin-resistant Staphylococcus aureus (MRSA) and Enterobacteriaceae expressing extended-spectrum β-lactamases (ESBL-E). The decontamination process preceded the conversion from piglet breeding to gilt production. Microbiological surveillance showed that the decontamination measures eliminated the MRSA and ESBL-E strains that were detected on the farm before the complete removal of pigs, cleaning and disinfection of the stable, and construction of an additional stable meeting high-quality standards. After pig production was restarted, ESBL-E remained undetectable over 12 months, but MRSA was recovered from pigs and the environment within the first 2 days. However, spa (Staphylococcus aureus protein A gene) typing revealed acquisition of an MRSA strain (type t034) that had not been detected before decontamination. Interestingly, we observed that a farmworker who had been colonized with the prior MRSA strain (t2011) acquired the new strain (t034) after 2 months. In summary, this report demonstrates that decontamination protocols similar to those used here can lead to successful elimination of contaminating MRSA and ESBL-E in pigs and the stable environment. Nevertheless, decontamination protocols do not prevent the acquisition of new MRSA strains.