Produção Nacional
Revisado por pares
First Report of Stem Canker Caused by Lasiodiplodia subglobosa on Carya illinoinensis in Brazil
2016; American Phytopathological Society; Volume: 100; Issue: 5 Linguagem: Inglês
10.1094/pdis-08-15-0948-pdn
ISSN1943-7692
AutoresTales Poletto, Caciara Gonzatto Maciel, Marlove Fátima Brião Muniz, Elena Blume, Igor Poletto, P. S. T. Brioso,
Tópico(s)Forest Insect Ecology and Management
ResumoHomePlant DiseaseVol. 100, No. 5First Report of Stem Canker Caused by Lasiodiplodia subglobosa on Carya illinoinensis in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Stem Canker Caused by Lasiodiplodia subglobosa on Carya illinoinensis in BrazilT. Poletto, C. Gonzatto Maciel, M. Muniz, E. Blume, I. Poletto, and P. BriosoT. PolettoSearch for more papers by this author, C. Gonzatto MacielSearch for more papers by this author, M. MunizSearch for more papers by this author, E. BlumeSearch for more papers by this author, I. PolettoSearch for more papers by this author, and P. BriosoSearch for more papers by this authorAffiliationsAuthors and Affiliations T. Poletto C. Gonzatto Maciel M. Muniz E. Blume I. Poletto , Universidade Federal de Santa Maria, Defesa Fitossanitária, Santa Maria, Brazil P. Brioso , Universidade Federal Rural do Rio de Janeiro, Entomologia e Fitopatologia, Rio de Janeiro, Brazil Published Online:4 Mar 2016https://doi.org/10.1094/PDIS-08-15-0948-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Pecan [Carya illinoinensis (Wangenh.) K. Koch], a member of the Juglandaceae, is important to the economy of southern Brazil. Stem canker symptoms were observed on 3-year-old trees of cultivars Desirable, Shawnee, Success, Jackson, and Barton in orchards located in Santa Maria (29°43′06″ S; 53°43′00″ W), Rio Grande do Sul State, Brazil. The disease resulted in 100% loss of symptomatic plants. Approximately half of the 2-ha orchard studied was infected. This disease can be a serious problem if disseminated. Initial symptoms appeared as small, elliptically-shaped, protruding lesions on the trunk bark and branches, progressing to cankers ranging from 6 to 60 cm and eventually leading to plant death. Necrosis and dark streaks were observed in longitudinal sections of the trunk, near the vascular tissue. Portions of symptomatic trunks and branches were surface disinfected with 70% ethanol for 3 min and 1% sodium hypochlorite for 5 min. Portions were rinsed 3× in sterile distilled water, placed in gearboxes, and incubated in a moist chamber at 25 ± 2°C with a 12-h photoperiod. After 3 days, fungal structures were observed. Fungal material was transferred first to potato dextrose agar (PDA) medium and subsequently to leaf-pine-agar (LPA) at 25 ± 2°C for 12 days to induce the production of reproductive structures. On PDA, the colony color ranged from dark gray to black. On LPA, the pathogen produced pycnidia covered with aerial mycelium. Conidia when young ranged from ovoid to subglobose, were hyaline, and had no septa. The conidia became dark brown with a median septum and presented longitudinal grooves, and were 17 to 25 × 12 to 16 μm (avg. 50 conidia). DNA extraction was performed employing the reagent CTAB as described by Doyle and Doyle (1991). A region of the elongation factor 1-α gene was amplified (PCR conditions: initial denaturation at 94°C/2 min; 40 cycles of 94°C/30s, 54°C/30s, 72°C/40s, final extension at 72°C/4 min) with primers EF1 and EF2 (O’Donnell and Cigelnik, 1997) and showed 100% similarity to Lasiodiplodia subglobosa (GenBank Accession No. KF226721) Accession No. KT895370. Based on morphological and molecular criteria the fungus was identified as L. subglobosa (Machado et al. 2014). A pathogenicity test to fulfill Koch’s postulate was performed on 1-year-old and 6-month-old pecan seedlings under greenhouse conditions. The inoculation of the fungus was performed on the stem using a 3-mm-diameter borer to prepare a hole 15 cm above ground. A 14-day-old plug (3-mm diameter) of PDA with the fungus was inserted in the hole and subsequently protected by wrapping the stem with a transparent plastic film. The control received a plug of PDA. Forty-five days after inoculation, the plants showed necrosis at the site of inoculation and at other isolated spots on the trunk; black streaks were observed in the vascular system, similar to those observed in the original infected sample plants. Subsequently, the pathogen was reisolated from the necrotic tissue and morphologically confirmed. Although this fungal species has previously been reported to cause collar and root rot on Jatropha curcas L. in Minas Gerais State, Brazil (Machado et al. 2014), this is the first report of L. subglobosa causing canker on C. illinoinensis in Brazil.References:Doyle, J. J., and Doyle, J. L. 1991. Focus 12:13. Google ScholarMachado, A. R., et al. 2014. Fungal Divers. 67:231. https://doi.org/10.1007/s13225-013-0274-1 Crossref, ISI, Google ScholarO’Donnell, K., and Cigelnik, E. 1997. Mol. Phylogenet. Evol. 7:103. https://doi.org/10.1006/mpev.1996.0376 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 5 May 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 15 Apr 2016Published: 4 Mar 2016First Look: 12 Jan 2016Accepted: 15 Dec 2015 Page: 1016 Information© 2016 The American Phytopathological SocietyCited byLasiodiplodia species diversity associated with coconut leaf blight and stem-end rot in Northeastern Brazil21 September 2021 | European Journal of Plant Pathology, Vol. 162, No. 1FUNGI OF THE BOTRYOSPHAERIACEAE FAMILY CAUSE DIFFERENT LEVELS OF STEM CANKER ON PECAN TREES (Carya illinoinensis) IN BRAZIL1 January 2022 | Revista Árvore, Vol. 46Botryosphaeriaceae species causing dieback on Annonaceae in Brazil1 July 2019 | Plant Pathology, Vol. 68, No. 7Phylogeny, Distribution, and Pathogenicity of Lasiodiplodia Species Associated With Cankers and Dieback Symptoms of Persian Lime in MexicoM. A. Bautista-Cruz, G. Almaguer-Vargas, S. G. Leyva-Mir, M. T. Colinas-León, K. C. Correia, M. Camacho-Tapia, L. Robles-Yerena, S. J. Michereff, and J. M. Tovar-Pedraza15 April 2019 | Plant Disease, Vol. 103, No. 6
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