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Separation of DNA Strands Facilitates Detection of Point Mutations by PCR-SSCP

1997; Future Science Ltd; Volume: 22; Issue: 4 Linguagem: Inglês

10.2144/97224bm05

1940-9818

Nagamiah Selvakumar, Bei-Chuan Ding, Stuart M. Wilson,

Plant and Fungal Interactions Research

BioTechniquesVol. 22, No. 4 BenchmarksOpen AccessSeparation of DNA Strands Facilitates Detection of Point Mutations by PCR-SSCPNagamiah Selvakumar, Bei-Chuan Ding & Stuart M. WilsonNagamiah SelvakumarTuberculosis Research Centre (ICMR), Madras, India, Bei-Chuan DingTuberculosis Research Centre (ICMR), Madras, India & Stuart M. Wilson*Address correspondence to Stuart M. Wilson, Deputy Head, Mycobacterium Reference Unit, PHLS Dulwich Hospital, East Dulwich Grove, London SE22 8QF, England, UK.Tuberculosis Research Centre (ICMR), Madras, IndiaPublished Online:7 Aug 2018https://doi.org/10.2144/97224bm05AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail "Separation of DNA Strands Facilitates Detection of Point Mutations by PCR-SSCP." , 22(4), pp. 604–606FiguresReferencesRelatedDetailsCited ByNanosensors for microbial detection in soilMethods for Exploring Soil Microbial Diversity25 May 2019Molecular tools for deciphering the microbial community structure and diversity in rumen ecosystem12 July 2012 | Applied Microbiology and Biotechnology, Vol. 95, No. 5Novel two-dimensional DNA gel electrophoresis mapping for characterizing complex bacterial communities in environmental samplesJournal of Bioscience and Bioengineering, Vol. 107, No. 6Genotypic Microbial Community Profiling: A Critical Technical Review8 March 2007 | Microbial Ecology, Vol. 54, No. 2Molecular Approaches for the Measurement of Density, Diversity, and Phylogeny14 May 2007Molecular Analysis of Cytokines and Cytokine Receptors10 March 2006Single-chain polymorphism analysis in long QT syndrome using planar waveguide fluorescent biosensorsAnalytical Biochemistry, Vol. 315, No. 2Increasing the Sensitivity of Single-Strand Conformation Polymorphism Analysis of the LDLR Gene Mutations in Brazilian Patients with Familial HypercholesterolemiaClinical Chemistry and Laboratory Medicine, Vol. 40, No. 5PCR Methods and ApproachesPCR-SSCP comparison of 16S rDNA sequence diversity in soil DNA obtained using different isolation and purification methodsFEMS Microbiology Ecology, Vol. 36, No. 2-3PCR-SSCP: A Method for Reliably Detecting Single Nucleotide Polymorphisms in Multifactorial DiseasesPolymerase chain reaction—single strand conformation polymorphism or how to detect reliably and efficiently each sequence variation in many samples and many genesElectrophoresis, Vol. 19, No. 18A New Approach To Utilize PCR–Single-Strand-Conformation Polymorphism for 16S rRNA Gene-Based Microbial Community AnalysisApplied and Environmental Microbiology, Vol. 64, No. 12Genotyping metabolic polymorphisms in a cohort of Caucasians and single strand conformation polymorphism analysis of point mutations in humanhprt exons 7 and 8Electrophoresis, Vol. 19, No. 14Comparison of different methods to produce single-strand DNA for identification of canned tuna by single-strand conformation polymorphism analysisElectrophoresis, Vol. 19, No. 8-9Automated fluorescent analysis procedure for enzymatic mutation detection1 April 1998 | Clinical Chemistry, Vol. 44, No. 4 Vol. 22, No. 4 Follow us on social media for the latest updates Metrics Downloaded 56 times History Published online 7 August 2018 Published in print April 1997 Information© 2018 Author(s)PDF download