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Prognostic significance of DNA cytometry for adjuvant therapy response in pancreatic cancer

2015; Elsevier BV; Volume: 15; Issue: 3 Linguagem: Inglês

10.1016/j.pan.2015.05.405

1424-3911

Fritz Klein, Marcus Bahra, Anja Schirmeier, Hussein Al‐Abadi, Johann Pratschke, Uwe Pelzer, Helmut Oettle, Jana K. Striefler, Hanno Riess, Marianne Sinn,

Cancer Cells and Metastasis

In this prospective study, an image cytomytric DNA-analysis was performed in 86 women with, breast neoplasms (72 primary invasive carcinomas and 14 benign lesions). Four DNA ploidy parameters were analysed: histogram type (according to AUER classification1), DNA-index, tumor cells wdb DNA content above the 5n limit and DNA malignancy grade (DNA-MG, calculation according to Böcking4,5,6,7). Their correlations witb well established prognostic factors in breast carcinomas (tumor size, lymph node status, histologic grade, hormone receptor content) were studied.All but one benign lesions were diploid (13/14 cases), whereas the majority of the primary invasive breast carcinomas were aneuploid (58/72 cases). A predominance of carcinomas witb a percentage of cells superior orequal to 1% with DNA content above the 5n limit was observed (54 cases out of 58). Most of the aneuploid tumors had a histogrom type III or IV (53 cases) or a high DNA-index (50 cases). Of these 58 aneuploid cases, only 26 tumors bad a DNA-MG superior to 1. Interestingly, 26 tumors had the 4 criteria of aneuploidy, 29 had 3 and 9 bud 2 and only 4 tumors bad one parameter.The DNA-MG was significantly related to hormonal receptors (p <0.001) and tumor size (p <0.01). The histogram types (Auer classification) and the DNA content above the 5n limit were correlated with hismlogic grade (SBR or SBRM) (p <0.02). Concerning the DNA-index no correlation was observed with well established prognostic factors. On the other hand no significant correlation was found between these new biologic variables and lymph node status. The “estimated S phase” sbowed a significant correlation between histogic grade (p <0.01) and PgR status (p <0.01).In conclusion, additional studies of larger groups of women and a long-term follow up are needed to see which variable or combination of variables will provide non-redundant prognostic information.