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Ltposome-Encapsulated Hemoglobin as an Artificial Red Blood Cell: Characterization and Scale-Up

1989; Marcel Dekker; Volume: 17; Issue: 5 Linguagem: Inglês

10.3109/10731198909117635

0890-5533

A. Vidal-Naquet, J. L. Gossage, Theresa Sullivan, J. W. Haynes, B. H. Gilruth, Richard L. Beissinger, Lalit Sehgal, Arthur L. Rosen,

Hemoglobin structure and function

Encapsulation methods using high pressure extrusion and homogenization were developed which produce hemoglobin-containing liposomes, approximately one micron or less in diameter with an oxygen-carrying capacity of more than half that of red blood cells (RBCs). These methods were developed for scaling-up liposome-encapsulated hemoglobin (LEH) production. Previously, the lack of adequate scale-up methods has been a serious barrier to full scale efficacy and toxicity testing for all the researchers engaged in such investigations. The scale-up methods and characterization of the resulting LEH preparations are presented.