Produção Nacional
Revisado por pares
PREVALÊNCIA DE ANTICORPOS ANTI-B. ovis E ANTI-B. abortus EM OVINOS DO MUNICÍPIO DE COLINAS, TOCANTINS, BRASIL
2013; UNIVERSIDADE FEDERAL DE GOIÁS; Volume: 42; Issue: 2 Linguagem: Inglês
10.5216/rpt.v42i2.25525
ISSN1980-8178
AutoresNekita Évely Ximenes Martins, Jefferson Douglas de Moura Almeida, Marco Giannoccaro Silva, Marlos Gonçalves Sousa, Luís Antônio Mathias, Katyane de Sousa Almeida,
Tópico(s)Leptospirosis research and findings
ResumoBrucellosis is an infectious disease caused by bacteria of the genus Brucella, responsible for reproductive disorders in animals, especially ruminants. This study aimed to determine the presence of antibodies against Brucella ovis and Brucella abortus in sheep of 14 registered properties of the Sheep Breeders Association in the city of Colinas, Tocantins, Brazil. Four hundred and fifty serum samples from ovines were collected, analyzed by rapid plate agglutination with acidified buffered antigen and when positive, were analyzed by the technique of slow agglutination in tubes (SAT) and 2-mercaptoethanol (2-ME), to search for smooth strains (Brucella abortus). The search for rough strains (Brucella ovis) was performed with agar gel immunodiffusion (AGID) and the samples that reacted were further tested by the complement fixation test (CFT). From the analyzed samples, 31.6% reacted in the AGID test. Among these, only 2.8% were confirmed positive by CFT. In the sera analyzed by rapid plate agglutination with acidified buffered antigen, 4.4% were positive, among them, 70% were confirmed positive by the 2-ME test. The results obtained in CFT and SAT/2-ME, when analyzed by the Fisher’s exact test and OR, showed statistical significance between positivity and age, showing that there was a greater chance of occurrence of brucellosis in a breeding animal.
Referência(s)