Proteomic analysis of a compatible interaction between sugarcane and Sporisorium scitamineum
2016; Wiley; Volume: 16; Issue: 7 Linguagem: Inglês
10.1002/pmic.201500245
ISSN1615-9861
AutoresLeonard Barnabas Ebinezer, N. M. R. Ashwin, K. Kaverinathan, Anna Rita Trentin, Micaela Pivato, A. Ramesh Sundar, P. Malathi, R. Viswanathan, Rosana Ottakandathil Babu, Neethukrishna Kausthubham, Paolo Carletti, Giorgio Arrigoni, Antonio Masi, Ganesh Kumar Agrawal, Randeep Rakwal,
Tópico(s)Natural Products and Biological Research
ResumoSmut caused by Sporisorium scitamineum is one of the important diseases of sugarcane with global significance. Despite the intriguing nature of sugarcane, S. scitamineum interaction, several pertinent aspects remain unexplored. This study investigates the proteome level alterations occurring in the meristem of a S. scitamineum infected susceptible sugarcane cultivar at whip emergence stage. Differentially abundant proteins were identified by 2DE coupled with MALDI-TOF/TOF-MS. Comprehensively, 53 sugarcane proteins identified were related to defence, stress, metabolism, protein folding, energy, and cell division; in addition, a putative effector of S. scitamineum, chorismate mutase, was identified. Transcript expression vis-à-vis the activity of phenylalanine ammonia lyase was relatively higher in the infected meristem. Abundance of seven candidate proteins in 2D gel profiles was in correlation with its corresponding transcript expression levels as validated by qRT-PCR. Furthermore, this study has opened up new perspectives on the interaction between sugarcane and S. scitamineum.
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