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The morphologic distribution of intravenously injected fatty chyle and artificial fat emulsion in rats and dogs.

1951; National Institutes of Health; Volume: 38; Issue: 1 Linguagem: Inglês

Raymond G. Murray, Smith Freeman,

Glycogen Storage Diseases and Myoclonus

Abstract The distribution of fat in certain organs of rats and dogs was studied primarily by frozen sections stained with oil red O, at intervals from ten minutes to twenty-four hours after rapid intravenous administration of 0.6 to 1.2 Gm. per kilogram of olive oil in emulsified form. This was compared with the distribution of fat following similarly injected fatty dog chyle as well as that of olive oil administered by stomach tube. Olive oil in the amount of 2 ml. given by stomach tube to rats could not be identified in spleen, liver, or lungs. The fat of injected artificial emulsions always appeared by ten minutes after injection in large amounts in the marginal zone of the splenic red pulp. In contrast, the fat of dog chyle was never concentrated here. Within ten minutes after injection of chyle, fat appeared in the parenchymal cells of the liver of dogs; at this interval after artificial emulsion, fat was concentrated in the Kupffer cells. The evidence from rat livers was clouded by variable fat content in the livers of untreated rats. Kupffer cells of rats contained fat only in recently anesthetized animals which had received emulsion; there was some evidence that this fat was passed on to the parenchymal cells. Chyle fat was never concentrated in the Kupffer cells. Lung capillaries of rats were packed with fat ten minutes after injections of artificial emulsion, but at subsequent intervals only scattered droplets remained in the septal macrophages. Chyle fat was never concentrated in the lung. Fat did not appear in significant quantities outside the blood vessels in cervical lymph nodes, kidney, heart, thymus, stomach, duodenum, or ileum after injections of either chyle or emulsion. The sheathed arteries of the dog spleen were sites of a striking concentration of fat in dogs receiving either emulsion or chyle. No concentration of fat could be detected in the rat spleen that would correspond to sheathed arteries. Possible causes of the considerable variation in results are discussed and the need for a labeled fat for future experiments is indicated.