Artigo Produção Nacional Revisado por pares

Diphenyl diselenide and sodium selenite associated with chemotherapy in experimental toxoplasmosis: influence on oxidant/antioxidant biomarkers and cytokine modulation

2014; Cambridge University Press; Volume: 141; Issue: 13 Linguagem: Inglês

10.1017/s0031182014001073

ISSN

1469-8161

Autores

CLEBER F. BARBOSA, Alexandre A. Tonin, Aleksandro S. Da Silva, María Isabel de Azevedo, Danieli Urach Monteiro, Emily Pansera Waczuk, Thiago Duarte, Carine Lima Hermes, Giovana Camillo, Fernanda F. Vogel, Luciana Faccio, Paula T. Tonin, Patrícia Wolkmer, Marta R. Leal, Marta Maria Medeiros Frescura Duarte, Rafael Noal Moresco, Sônia T.A. Lopes, Mário Luiz de la Rue,

Tópico(s)

Sulfur Compounds in Biology

Resumo

SUMMARY The aim of this study was to assess the effect of sulfamethoxazole/trimethoprim (ST) supplemented with diphenyl diselenide and sodium selenite in experimental toxoplasmosis, on oxidant/antioxidant biomarkers and cytokine levels. Eighty-four BALB/c mice were divided in seven groups: group A (negative control), and groups B to G (infected). Blood and liver samples were collected on days 4 and 20 post infection (p.i.). Levels of thiobarbituric acid (TBA) reactive substances and advanced oxidation protein products (AOPP) were assessed in liver samples. Both biomarkers were significantly increased in infected groups on day 4 p.i., while they were reduced on day 20 p.i., compared with group A. Glutathione reductase (GR) activity significantly (P<0·01) increased on day 4 p.i., in group G, compared with group A. INF-γ was significantly increased (P<0·001) in both periods, day 4 (groups B, C, F and G) and 20 p.i. (groups C, F and G). IL-10 significantly reduced (P<0·001) on day 4 p.i. in group B; however, in the same period, it was increased (P<0·001) in groups C and G, compared with group A. On day 20 p.i., IL-10 increased (P<0·001) in groups F and G. Therefore, our results highlighted that these forms of selenium, associated with the chemotherapy, were able to reduce lipid peroxidation and protein oxidation, providing a beneficial immunological balance between the production of pro- and anti-inflammatory cytokines.

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