Functional genetics of Yarrowia lipolytica
2003; Springer Science+Business Media; Linguagem: Inglês
10.1007/3-540-37003-x_8
ISSN1610-6970
AutoresGerold Barth, Jean-Marie Beckerich, Ángel Domínguez, Stefan Kerscher, David M. Ogrydziak, Vladimir I. Titorenko, Claude Gaillardin,
Tópico(s)Biofuel production and bioconversion
ResumoThe yeast Yarrowia lipolytica has been used for industrial bioconversions since the late 1960's, mainly by the food and chemical industries. The most important process is presently the production of citric acid from rapeseed oil, but Y. lipolytica has also been considered for various other applications, ranging from single cell protein to heterologous proteins production. Several of these procedures have received a GRAS status, which facilitates further applications of this yeast in the food and pharmaceutical industries. This review focuses on the acquisition of basic knowledge on Y. lipolytica, which is physiologically and taxonomically very distant from Saccharomyces cerevisiae. Thanks to the development of advanced genetic tools for both classical approaches and recombinant DNA technology, significant progresses have been achieved in several aspects of its unusual biology. Particular attention has been paid to the study of early steps of protein secretion, peroxisome biogenesis, carbon metabolism and utilisation of hydrophobic substrates, mitochondrial biology, morphogenetic control, and proteae regulation. An international effort relayed by the Genoscope will permit access to its full genome sequence by the end of 2003, thus facilitating further developments of its functional genetics.
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