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Clinical events and their relation to the tumor necrosis factor-alpha and interleukin-10 genotypes in Sickle-Cell-Anemia patients

2015; Elsevier BV; Volume: 9; Issue: 1 Linguagem: Inglês

10.1016/j.hemonc.2015.11.002

1658-3876

Jaâio Emanuel Andrade Cavalcante, Rosângela Pinheiro Gonçalves Machado, Marília Rocha Laurentino, Talyta Ellen de Jesus dos Santos, Izabel Cristina Justino Bandeira, Pedro Aurio Maia Filho, Monalisa Feliciano Figueiredo, Alice Maria Costa Martins, Romélia Pinheiro Gonçalves Lemes,

Iron Metabolism and Disorders

Objective/background: Sickle-cell anemia (SCA) is a genetic blood disease characterized by chronic inflammation and a heterogeneous clinical picture. Serum tumor necrosis factor (TNF-alpha) and interleukin 10 (IL-10) levels are associated with the clinical course of SCA. This study aimed to evaluate the association between the frequency of the polymorphisms TNF-alpha- 308 G . A, IL-10-1082 G . A, IL-10-819 C . T, and IL-10-592 A . C; serum TNF-alpha; and IL-10 levels, and the incidence of clinical events in SCA patients. Methods: Polymerase chain reaction-restriction fragment length polymorphism and enzymelinked immunosorbent assay were performed on 25 adults with SCA at the steady state; their data were compared with those for 26 healthy individuals. Results: The most frequent genotype of the TNF-alpha polymorphism was GG (low producer), and the most frequent genotype of the IL-10 polymorphisms was “low producer” (ACC ACC, ACC ATA, ATA ATA). The TNF-alpha levels were significantly higher in SCA in patients with acute chest syndrome (ACS). The IL-10 levels were reduced in polytransfusion and in patients with ACS. Conclusion: The patients presented prevalence of TNF-alpha and IL-10 low-profile producer. The cytokine serum levels presented an association with the presence of polytransfusion and ACS in SCA patients.