Portal de Periódicos da CAPES

Granulocyte macrophage-colony stimulating factor stimulates both association and activation of phosphoinositide 3OH-kinase and src-related tyrosine kinase(s) in human myeloid derived cells.

1993; Springer Nature; Volume: 12; Issue: 7 Linguagem: Inglês

10.1002/j.1460-2075.1993.tb05929.x

1460-2075

Seth J. Corey, A. Eguinoa, K. Puyana-Theall, Joseph B. Bolen, Lewis C. Cantley, Faustino Mollinedo, Trevor Jackson, Phillip T. Hawkins, Len Stephens,

Cell Adhesion Molecules Research

Research Article1 July 1993free access Granulocyte macrophage-colony stimulating factor stimulates both association and activation of phosphoinositide 3OH-kinase and src-related tyrosine kinase(s) in human myeloid derived cells. S. Corey S. Corey Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author A. Eguinoa A. Eguinoa Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author K. Puyana-Theall K. Puyana-Theall Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author J.B. Bolen J.B. Bolen Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author L. Cantley L. Cantley Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author F. Mollinedo F. Mollinedo Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author T.R. Jackson T.R. Jackson Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author P.T. Hawkins P.T. Hawkins Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author L.R. Stephens L.R. Stephens Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author S. Corey S. Corey Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author A. Eguinoa A. Eguinoa Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author K. Puyana-Theall K. Puyana-Theall Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author J.B. Bolen J.B. Bolen Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author L. Cantley L. Cantley Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author F. Mollinedo F. Mollinedo Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author T.R. Jackson T.R. Jackson Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author P.T. Hawkins P.T. Hawkins Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author L.R. Stephens L.R. Stephens Department of Pediatrics, University of Pittsburgh, PA 15213. Search for more papers by this author Author Information S. Corey1, A. Eguinoa1, K. Puyana-Theall1, J.B. Bolen1, L. Cantley1, F. Mollinedo1, T.R. Jackson1, P.T. Hawkins1 and L.R. Stephens1 1Department of Pediatrics, University of Pittsburgh, PA 15213. The EMBO Journal (1993)12:2681-2690https://doi.org/10.1002/j.1460-2075.1993.tb05929.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The signalling pathways used by the GM-CSF receptor are currently unknown. Here we show that in human myeloid derived cells GM-CSF can stimulate; (i) the accumulation of PtdIns(3,4,5)P3; (ii) increases in p53/p56lyn and p62c-yes directed protein tyrosine kinase activities in anti-lyn and anti-c-yes antibody directed immunoprecipitates, respectively and; (iii) increases in phosphoinositide 3OH-kinase activity in antiphosphotyrosine, anti-p53/p56lyn and anti-p62c-yes antibody directed immunoprecipitates. These results suggest that GM-CSF can stimulate formation of protein tyrosine kinase co-ordinated signalling complexes, that contain p53/p56lyn, p62c-yes and an activated PtdInsP2 directed phosphoinositide 3OH-kinase, which can drive the accumulation of the putative second-messenger PtdIns(3,4,5)P3. Previous ArticleNext Article Volume 12Issue 71 July 1993In this issue RelatedDetailsLoading ...