Portal de Periódicos da CAPES

Protein Structure and Function Prediction Using I‐TASSER

2015; Wiley; Volume: 52; Issue: 1 Linguagem: Inglês

10.1002/0471250953.bi0508s52

1934-340X

Jianyi Yang, Yang Zhang,

RNA and protein synthesis mechanisms

I-TASSER is a hierarchical protocol for automated protein structure prediction and structure-based function annotation. Starting from the amino acid sequence of target proteins, I-TASSER first generates full-length atomic structural models from multiple threading alignments and iterative structural assembly simulations followed by atomic-level structure refinement. The biological functions of the protein, including ligand-binding sites, enzyme commission number, and gene ontology terms, are then inferred from known protein function databases based on sequence and structure profile comparisons. I-TASSER is freely available as both an on-line server and a stand-alone package. This unit describes how to use the I-TASSER protocol to generate structure and function prediction and how to interpret the prediction results, as well as alternative approaches for further improving the I-TASSER modeling quality for distant-homologous and multi-domain protein targets.