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SIMcheck: a Toolbox for Successful Super-resolution Structured Illumination Microscopy

2015; Nature Portfolio; Volume: 5; Issue: 1 Linguagem: Inglês

10.1038/srep15915

2045-2322

Graeme Ball, Justin Demmerle, Rainer Kaufmann, Ilan Davis, Ian M. Dobbie, Lothar Schermelleh,

Optical Coherence Tomography Applications

Abstract Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck , a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data and assess resolution and data quality through objective control parameters. Additionally, SIMcheck provides advanced calibration tools and utilities for common image processing tasks. This open-source software is applicable to all commercial and custom platforms and will promote routine application of super-resolution SIM imaging in cell biology.