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N-terminus of α-crystallin

1968; Elsevier BV; Volume: 7; Issue: 2 Linguagem: Inglês

10.1016/s0014-4835(68)80080-6

1096-0007

Herman J. Hoenders, J.G.G. Schoenmakers, Johannes Gerding, G. I. Tesser, H. Bloemendal,

Biochemical effects in animals

A pure N-terminal peptide was isolated from bovine lens α-crystallin after pronase digestion, cation exchange on Dowex 50W-X2 and two additional electrophoretic purification steps. The elucidation of the structure by means of high voltage electrophoresis, thin layer chromatography, gas chromatography and nuclear magnetic resonance spectroscopy resulted in the formula: N-acetyl-Met-Asp. One acetyl group was found per approximately 21,000 g of α-crystallin. The latter value is consistent with the molecular weight of the subunits. The previous findings that glutamic acid was the N-terminal amino acid must have been caused by contamination.