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Disposable screen printed sensor for the electrochemical detection of delta-9-tetrahydrocannabinol in undiluted saliva

2016; BioMed Central; Volume: 10; Issue: 1 Linguagem: Inglês

10.1186/s13065-016-0148-1

1752-153X

Ceri Wanklyn, D.P. Burton, Emma Enston, Carrie-Ann Bartlett, Sarah Taylor, Aleksandra Raniczkowska, Murdo Black, Lindy J. Murphy,

Neuroscience and Neuropharmacology Research

Cannabis has an adverse effect on the ability to drive safely, therefore a rapid disposable test for Δ(9)-tetrahydrocannabinol (Δ(9)-THC), the psychoactive component of cannabis, is highly desirable for roadside testing.A screen printed carbon electrode is used for the N-(4-amino-3-methoxyphenyl)-methanesulfonamide mediated detection of Δ(9)-THC in saliva. Mediator placed in an overlayer was galvanostatically oxidized and reacted with Δ(9)-THC to give an electrochemically active adduct which could be detected by chronoamperometric reduction. Detection of 25-50 ng/mL Δ(9)-THC spiked into undiluted saliva was achieved with a response time of 30 s. A trial of the sensors with four cannabis smokers showed sensitivity of 28 %, specificity of 99 % and accuracy of 52 %.Rapid electrochemical detection of Δ(9)-THC in undiluted saliva has been demonstrated using a disposable sensor, however the sensitivity is lower than acceptable. Further optimization of the assay and sensor format is required to improve the sensitivity of response to Δ(9)-THC.