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ANTIMICROBIAL POTENTIAL OF STREPTOMYCES CHEONANENSIS VUK-A FROM MANGROVE ORIGIN

2016; Innovare Academic Sciences; Volume: 8; Issue: 3 Linguagem: Inglês

2656-0097

Krishna Naragani, Ushakiranmayi Mangamuri, Vijayalakshmi Muvva, P. Sudhakar, Rajesh Kumar Munaganti,

Genomics and Phylogenetic Studies

Objectives: The aim of the present study was to isolate, characterize and evaluate the activity of compounds produced by Streptomyces cheonanensis VUK-A. Methods: Chemical examination of the secondary metabolites of the strain Streptomyces cheonanensis VUK-A has led to the segregation of one bioactive compound (1) and a partially purified fraction (2). The strain was isolated from the sediment samples of mangrove ecosystem of Coringa, south coastal Andhra Pradesh, India. The chemical structure of the active compound 1 was established on the basis of spectroscopic analysis including 1 H NMR, 13 C NMR spectroscopy, FTIR and EIMS. The partially purified sub-fraction (2) subjected to Gas Chromatography-Mass spectroscopy. The antimicrobial activity of the bioactive compounds produced by the strain was expressed in terms of minimum inhibitory concentration. Results: The compound 1 was isolated from the fermentation broth was characterized as benzoic acid (1) based on spectroscopic analysis. The partially purified sub-fraction (2) subjected to Gas Chromatography-Mass spectroscopy contained nine analogues: 1-tetradecene, tetradecane, 1-hexadecene, hexadecane, 5-octadecene, octadecane, 5-eicosene, 1-nonadecene and cyclo tetracosane. The compounds recorded moderate to significant antimicrobial activity against medicinally and agriculturally important bacteria and fungi. This is the first report of six partially purified compounds 1-tetradecene, tetradecane, hexadecane, octadecane, 5-eicosene and cyclo tetracosane from the genus Streptomyces. Conclusion: The results of the present study showed that the metabolites of Streptomyces cheonanensis VUK-A exhibited antibacterial and antifungal activities. The study also supports that Coringa, a promising mangrove ecosystem remained to be explored for new bioactive compounds. Keywords: Streptomyces cheonanensis , Mangrove Ecosystem, Natural Products, Antimicrobial activity.