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Histologic evaluation of Ki-67 and cleaved caspase-3 expression in feline mammary carcinoma

2016; SAGE Publishing; Volume: 19; Issue: 4 Linguagem: Inglês

10.1177/1098612x16634150

1532-2750

Millena N Silva, Juliana da Silva Leite, Marcela FV Mello, Kassia VGC Silva, Kátia Barão Corgozinho, Heloisa JM. Souza, Simone Carvalho dos Santos Cunha, Ana MR Ferreira,

Microbial infections and disease research

Objectives The purpose of this study was to evaluate histopathologic aspects of, and the expression of Ki-67 and cleaved caspase-3 in, feline mammary carcinoma (FMC). Methods Feline mammary tumors were surgically obtained by mastectomy from 30 female cats and were fixed with formalin and embedded in paraffin wax. Four-micron sections were stained with hematoxylin and eosin for histopathologic diagnosis. Ki-67 and cleaved caspase-3 were analyzed by immunohistochemistry. Results Samples were histologically confirmed as FMC. Positive immunostaining was observed in all cancer samples for both nuclear Ki-67 and cleaved caspase-3, with a mean positive staining percentage of 27.5% and 21.2%, respectively. No statistically significant correlations between Ki-67 and cleaved caspase-3 were observed within FMC. Conclusions and relevance A high proliferation index was found in feline mammary tumors. This is the first study evaluating cleaved caspase-3 expression in FMC.