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Differentiation of B. Coli and B. Aerogens on a Simplified Eosin-Methylene Blue Agar

1918; Oxford University Press; Volume: 23; Issue: 1 Linguagem: Inglês

10.1086/infdis/23.1.43

1537-6613

Max Levine,

Hydraulic Fracturing and Reservoir Analysis

For confirming the presumptive test for B. coli the mediums most frequently employed are litmus lactose agar and fuchsin sulphite (Endo) agar. It is becoming more apparent that the coli-like forms may be divided into two groups which are closely correlated with the source. One group (B. coli) is characteristic of fecal origin; the other (B. aerogenes and B. cloacae) is rare in feces, but constitutes the prevailing coli-like form in the soil and on grains. The standard litmus lactose and Endo agar may be employed to a slight extent for the differentiation of B. coli and B. aerogenes, but the differences between these types on these mediums (particularly L.L.A.) are not very clear-cut nor distinct. Better results are obtained with a modified Endo agar described elsewhere. A very excellent differentiation between the B. coli and B. aerogenes types has been obtained on a modification of eosin-methylene blue agar first described by HoltHarris and Teague for the isolation of the typhoid group from feces. The medium is prepared in the following manner: