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Synthesis of an ochre suppressor tRNA gene and expression in mammalian cells.

1984; Springer Nature; Volume: 3; Issue: 11 Linguagem: Inglês

10.1002/j.1460-2075.1984.tb02154.x

1460-2075

Frank A. Laski, R Belagaje, Robert M. Hudziak, Mario R. Capecchi, Grenville Norton, Peter Palese, Uttam L. RajBhandary, Phillip A. Sharp,

RNA Research and Splicing

Research Article1 November 1984free access Synthesis of an ochre suppressor tRNA gene and expression in mammalian cells. F.A. Laski F.A. Laski Search for more papers by this author R. Belagaje R. Belagaje Search for more papers by this author R.M. Hudziak R.M. Hudziak Search for more papers by this author M.R. Capecchi M.R. Capecchi Search for more papers by this author G.P. Norton G.P. Norton Search for more papers by this author P. Palese P. Palese Search for more papers by this author U.L. RajBhandary U.L. RajBhandary Search for more papers by this author P.A. Sharp P.A. Sharp Search for more papers by this author F.A. Laski F.A. Laski Search for more papers by this author R. Belagaje R. Belagaje Search for more papers by this author R.M. Hudziak R.M. Hudziak Search for more papers by this author M.R. Capecchi M.R. Capecchi Search for more papers by this author G.P. Norton G.P. Norton Search for more papers by this author P. Palese P. Palese Search for more papers by this author U.L. RajBhandary U.L. RajBhandary Search for more papers by this author P.A. Sharp P.A. Sharp Search for more papers by this author Author Information F.A. Laski, R. Belagaje, R.M. Hudziak, M.R. Capecchi, G.P. Norton, P. Palese, U.L. RajBhandary and P.A. Sharp The EMBO Journal (1984)3:2445-2452https://doi.org/10.1002/j.1460-2075.1984.tb02154.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info We have used site-specific mutagenesis to change the anticodon of a Xenopus laevis tyrosine tRNA gene so that it would recognize ochre codons. This tRNA gene is expressed when amplified in monkey cells as part of a SV40 recombinant and efficiently suppresses termination at both the ochre codon separating the adenovirus 2 hexon gene from a 23-kd downstream gene and the ochre codon at the end of the NS1 gene of influenza virus A/Tex/1/68. Termination at an amber codon of a NS1 gene of another influenza virus strain was not suppressed by the (Su+) ochre gene suggesting that in mammalian cells amber codons are not recognized by ochre suppressor tRNAs. Finally, microinjection into mammalian cells of both (Su+) ochre tRNA genes and selectible genes containing ochre nonsense mutations gives rise to colonies under selective conditions. We conclude that it should be possible to isolate a wide assortment of mammalian cell lines with ochre suppressor activity. Previous ArticleNext Article Volume 3Issue 111 November 1984In this issue RelatedDetailsLoading ...