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Inverted repeats in the long-terminal repeats of the wheat retrotransposon Wis 2-1A.

1992; Oxford University Press; Linguagem: Inglês

10.1093/oxfordjournals.molbev.a040742

1537-1719

Hélène Lucas, Graham Moore, George J. Murphy, R. B. Flavell,

Plant Disease Resistance and Genetics

The wheat insertion sequence Wis 2-1A possesses all the structural features characteristic of retrotransposons. Its long-terminal repeats (LTRs) are unusually long (1,755 bp) compared with those of other retrotransposons. Sequence analysis revealed that they differ from each other by only six point mutations. They contain a few tandem direct repeats, which could be explained by slippage mechanisms during replication. Almost half (44%) of the length of the LTRs is occupied by hairpin structures, which may relate to their large size. Possible origins of these inverted repeats are proposed, including the insertion and imprecise excision of transposable elements and errors when the DNA replication intermediate switches RNA template during retrotransposon replication.