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New Insights Into the Transmissibility of Leishmania infantum From Dogs to Sand Flies: Experimental Vector-Transmission Reveals Persistent Parasite Depots at Bite Sites

2016; Oxford University Press; Volume: 213; Issue: 11 Linguagem: Inglês

10.1093/infdis/jiw022

1537-6613

Hamide Aslan, Fabiano Oliveira, Claudio Meneses, Philip Castrovinci, Régis Gomes, Clarissa Teixeira, Candace A. Derenge, Marlene S. Orandle, Luigi Gradoni, Gætano Oliva, Laurent Fischer, Jesús G. Valenzuela, Shaden Kamhawi,

Trypanosoma species research and implications

Canine leishmaniasis (CanL) is a chronic fatal disease of dogs and a major source of human infection through propagation of parasites in vectors. Here, we infected 8 beagles through multiple experimental vector transmissions with Leishmania infantum–infected Lutzomyia longipalpis. CanL clinical signs varied, although live parasites were recovered from all dog spleens. Splenic parasite burdens correlated positively with Leishmania-specific interleukin 10 levels, negatively with Leishmania-specific interferon γ and interleukin 2 levels, and negatively with Leishmania skin test reactivity. A key finding was parasite persistence for 6 months in lesions observed at the bite sites in all dogs. These recrudesced following a second transmission performed at a distal site. Notably, sand flies efficiently acquired parasites after feeding on lesions at the primary bite site. In this study, controlled vector transmissions identify a potentially unappreciated role for skin at infectious bite sites in dogs with CanL, providing a new perspective regarding the mechanism of Leishmania transmissibility to vector sand flies.