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Rapid Bacterial Identification, Resistance, Virulence and Type Profiling using Selected Reaction Monitoring Mass Spectrometry

2015; Nature Portfolio; Volume: 5; Issue: 1 Linguagem: Inglês

10.1038/srep13944

2045-2322

Yannick Charretier, Olivier Dauwalder, Christine Franceschi, Elodie Degout-Charmette, Gilles Zambardi, Tiphaine Cecchini, Chloé Bardet, Xavier Lacoux, Philippe Dufour, Laurent Véron, Hervé Rostaing, Véronique Lanet, Tanguy Fortin, Corinne Beaulieu, Nadine Perrot, Dominique Dechaume, Sylvie Pons, Victoria Girard, Arnaud Salvador, G. Durand, Frédéric Mallard, Alain Théretz, Patrick Broyer, Sonia Chatellier, Gaspard Gervasi, Marc van Nuenen, Carolyn Roitsch, Alex van Belkum, Jérôme Lemoine, François Vandenesch, Jean‐Philippe Charrier,

Antibiotic Resistance in Bacteria

Mass spectrometry (MS) in Selected Reaction Monitoring (SRM) mode is proposed for in-depth characterisation of microorganisms in a multiplexed analysis. Within 60-80 minutes, the SRM method performs microbial identification (I), antibiotic-resistance detection (R), virulence assessment (V) and it provides epidemiological typing information (T). This SRM application is illustrated by the analysis of the human pathogen Staphylococcus aureus, demonstrating its promise for rapid characterisation of bacteria from positive blood cultures of sepsis patients.