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Mobilization of quiet, endogenous Tc3 transposons of Caenorhabditis elegans by forced expression of Tc3 transposase.

1993; Springer Nature; Volume: 12; Issue: 6 Linguagem: Inglês

10.1002/j.1460-2075.1993.tb05906.x

1460-2075

Henri G.A.M. van Luenen, Sean D. Colloms, Ronald H.A. Plasterk,

Chromosomal and Genetic Variations

Research Article1 June 1993free access Mobilization of quiet, endogenous Tc3 transposons of Caenorhabditis elegans by forced expression of Tc3 transposase. H.G. van Luenen H.G. van Luenen Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam. Search for more papers by this author S.D. Colloms S.D. Colloms Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam. Search for more papers by this author R.H. Plasterk R.H. Plasterk Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam. Search for more papers by this author H.G. van Luenen H.G. van Luenen Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam. Search for more papers by this author S.D. Colloms S.D. Colloms Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam. Search for more papers by this author R.H. Plasterk R.H. Plasterk Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam. Search for more papers by this author Author Information H.G. Luenen1, S.D. Colloms1 and R.H. Plasterk1 1Netherlands Cancer Institute, Division of Molecular Biology, Amsterdam. The EMBO Journal (1993)12:2513-2520https://doi.org/10.1002/j.1460-2075.1993.tb05906.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info The commonly studied Caenorhabditis elegans strain Bristol N2 contains approximately 15 copies per genome of the transposon Tc3. However, Tc3 is not active in Bristol N2. Tc3 contains one major open reading frame (Tc3A). We have fused this open reading frame to an inducible promoter and expressed it in a transgenic Bristol N2 line. Tc3A expression resulted in frequent excision and transposition of endogenous Tc3 elements. This shows that the Bristol N2 genome contains Tc3 transposons that are cis proficient for transposition, but are immobile because Tc3A is absent. We demonstrate that recombinant Tc3A binds specifically to the terminal nucleotides of the Tc3 inverted repeat, indicating that Tc3A is the Tc3 transposase. Activation of Tc3 transposition in vivo was accompanied by the appearance of extrachromosomal, linear copies of Tc3. These may be intermediates in Tc3 transposition. Previous ArticleNext Article Volume 12Issue 61 June 1993In this issue RelatedDetailsLoading ...