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BIBTEX RIS

Species-level resolution of 16S rRNA gene amplicons sequenced through the MinION™ portable nanopore sequencer

2016; University of Oxford; Volume: 5; Issue: 1 Linguagem: Inglês

10.1186/s13742-016-0111-z

ISSN

2047-217X

Autores

Alfonso Benı́tez-Páez, Kevin Portune, Yolanda Sanz,

Tópico(s)

Environmental DNA in Biodiversity Studies

Resumo

BackgroundThe miniaturised and portable DNA sequencer MinION™ has been released to the scientific community within the framework of an early access programme to evaluate its application for a wide variety of genetic approaches. This technology has demonstrated great potential, especially in genome-wide analyses. In this study, we tested the ability of the MinION™ system to perform amplicon sequencing in order to design new approaches to study microbial diversity using nearly full-length 16S rDNA sequences.

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