Leptospira pomona Infection in a Woodchuck: Preliminary Report
1960; Volume: 75; Issue: 10 Linguagem: Inglês
10.2307/4590959
ISSN2327-6258
AutoresLawrence G. Clark, J. I. Kresse, Robert R. Marshak, Charles J. Hollister,
Tópico(s)Mycobacterium research and diagnosis
ResumoIn an epizootiological study of leptospirosis and brucellosis in cattle and wildlife in Chester County, Pa., Leptospira pomona was isolated from a southeastern woodchuck, Marniota mo- nax monaa (1).This was the first time L. po- qmona had been isolated from this species in the United States.Infection with L. pomona is common among cattle in southeastern Pennsylvania. Materials and MethodsKidney and-urine specimens were removed aseptically.The kidney was ground with mor- tar and pestle and a 10 percent by volume suspension was made with Stuart's base liquid medium (Difco).The following media were employed: Fletcher's semisolid medium (Difco) containing 10 percent rabbit serum; Fletcher's semisolid medium containing 15 per- cent horse serum; Chang's semisolid medium (2), Hamilton, Mont., modification (3), con- taining 10 percent rabbit serum; Chang's semi- solid medium containing 15 percent horse se- rum; and Stuart's semisolid medium (4) containing 10 percent rabbit serum.One set. of media tubes was inoculated with 2-3 drops of kidney suspension and one set was inoculated with 1-2 drops of urine.Serial tenfold dilu- tions of kidney suspension were then made, with Stuart's base liquid medium, to approximate 10-2, 10-3 and 104 final dilutions.A 0.1-cc.inoculum of each of these dilutions was streaked on petri plates containing 30-35 cc. of Cox's (5) plate medium.Tubes and plates were in-
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