Portal de Periódicos da CAPES

First Report of Tomato chlorotic spot virus in Scarlet Eggplant ( Solanum aethiopicum ) and American Black Nightshade ( Solanum americanum ) in the United States

2015; American Phytopathological Society; Volume: 99; Issue: 10 Linguagem: Inglês

10.1094/pdis-04-15-0385-pdn

1943-7692

Ismael E. Badillo-Vargas, Nancy E. Roe, J. E. Funderburk, Scott Adkins,

Plant Disease Management Techniques

HomePlant DiseaseVol. 99, No. 10First Report of Tomato chlorotic spot virus in Scarlet Eggplant (Solanum aethiopicum) and American Black Nightshade (Solanum americanum) in the United States PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Tomato chlorotic spot virus in Scarlet Eggplant (Solanum aethiopicum) and American Black Nightshade (Solanum americanum) in the United StatesI. E. Badillo-Vargas, N. Roe, J. E. Funderburk, and S. AdkinsI. E. Badillo-Vargas, N. Roe, J. E. Funderburk, and S. AdkinsAffiliationsAuthors and Affiliations I. E. Badillo-Vargas , University of Florida, NFREC, Quincy, FL 32351 N. Roe , Farming Systems Research, Inc., Boynton Beach, FL 33474 J. E. Funderburk , University of Florida, NFREC, Quincy, FL 32351 S. Adkins , USDA-ARS, Fort Pierce, FL 34945. Published Online:24 Aug 2015https://doi.org/10.1094/PDIS-04-15-0385-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Scarlet eggplant (also known as Ethiopian nightshade or Brazilian eggplant) originated in sub-Saharan Africa and is now grown in many parts of the world as a food, medicinal, or ornamental crop, as well as for use as a rootstock for tomato and common eggplant (Solanum melongena) (Sakhanokho et al. 2014). American black nightshade is a common solanaceous weed in Florida and many subtropical and tropical areas of the world, and has been identified as a natural reservoir host for two tospoviruses [Tomato spotted wilt virus (TSWV) and Groundnut ringspot virus (GRSV)] and an experimental host for a third [Tomato chlorotic spot virus (TCSV)] (Webster et al. 2015). During January and February 2015, two scarlet eggplant and one American black nightshade plants were observed with virus-like symptoms typical of tospoviruses on a vegetable farm in southeast Florida (Palm Beach County). Both TCSV and its western flower thrips (Frankliniella occidentalis) vector were previously detected on tomato and pepper at this farm. A general foliar chlorosis and yellow-orange spotting of mature fruits were observed on the scarlet eggplant samples, and chlorotic foliar ringspots and ring patterns were observed on the American black nightshade (Supplemental file). Samples of all three plants reacted with commercially available TSWV lateral flow immunoassay reagents (Agdia, Inc., Elkhart, IN), indicating the presence of one or more tospoviruses. Total RNA was extracted (RNeasy Plant Mini Kit, Qiagen, Valencia, CA) from symptomatic tissue of these three plant samples and tested for TSWV, GRSV, and TCSV by RT-PCR as previously described (Webster et al. 2015). Primers specific for the TCSV RNA-dependent RNA polymerase (L) gene (TCSV-3′Lv/TCSV-3′Lvc; 867 bp), nonstructural movement protein (NSm) gene (TCSV-NSmv/TCSV-NSmvc; 835 bp), or nucleocapsid (N) gene (TCSV-Nv2/TCSV-Nvc2; 478 bp) amplified products of the expected size. Primers specific for the N gene of TSWV (Adkins and Rosskopf 2002) or GRSV (Webster et al. 2011) did not amplify products from any sample. TCSV amplicons from one scarlet eggplant sample and the American black nightshade sample were cloned in the pGEM-T vector (Promega, Madison, WI). Five clones of each amplicon were sequenced in both directions and consensus sequences were deposited in GenBank (Accession Nos. KR012984 to KR012989). Sequence analysis demonstrated that all three genes from both plant species shared >98% nucleotide identity with TCSV isolates in GenBank, including previously characterized isolates from tomato, long bean, chili pepper, pepper, jimsonweed, and lettuce in Florida, Dominican Republic, and/or Puerto Rico. TCSV has been previously identified from scarlet eggplant in Paraiba Valley, Sao Paulo, Brazil (Eiras et al. 2002) but, to the best of our knowledge, this is the first report of TCSV naturally infecting scarlet eggplant and American black nightshade in the United States. Expansion of the TCSV host range in the United States provides additional opportunities for spread of this emerging virus to crops of economic importance and potential reservoir species in Florida and elsewhere.References:Adkins, S., and Rosskopf, E. N. 2002. Plant Dis. 86:1310. https://doi.org/10.1094/PDIS.2002.86.12.1310 Link, ISI, Google ScholarEiras, M., Chaves, A. L. R., Colariccio, A., Harakava, R., De Araujo, J., and Chagas, C. M. 2002. Fitopatol. Bras. 27:285. https://doi.org/10.1590/S0100-41582002000300008 Crossref, Google ScholarSakhanokho, H. F., Islam-Faridi, M. N., Blythe, E. K., Smith, B. J., Rajasekaran, K., and Majid, M. A. 2014. J. Crop Improv. 28:437. https://doi.org/10.1080/15427528.2014.913280 Crossref, Google ScholarWebster, C. G., Reitz, S. R., Perry, K. L., and Adkins, S. 2011. Virology 413:216. https://doi.org/10.1016/j.virol.2011.02.011 Crossref, ISI, Google ScholarWebster, C. G., Frantz, G., Reitz, S. R., Funderburk, J. E., Mellinger, H. C., McAvoy, E., Turechek, W. W., Marshall, S. H., Tantiwanich, Y., McGrath, M. T., Daughtrey, M. L., and Adkins, S. 2015. Phytopathology 105:388. https://doi.org/10.1094/PHYTO-06-14-0172-R Link, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 99, No. 10 October 2015SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 5 Oct 2015Published: 24 Aug 2015First Look: 11 May 2015Accepted: 1 May 2015 Page: 1450 Information© 2015 The American Phytopathological SocietyCited byTomato chlorotic spot virusCABI Compendium, Vol. CABI CompendiumSolanum aethiopicum (African eggplant / Scarlet eggplant)6 June 2020Solanum americanum (American black nightshade)6 June 2020Viruses of ornamentals emerging in Florida and the Caribbean regionActa Horticulturae, No. 1193Integration of chitosan and plant growth-promoting rhizobacteria to control Papaya ringspot virus and Tomato chlorotic spot virus10 December 2017 | Archives of Phytopathology and Plant Protection, Vol. 50, No. 19-20First Report of Tomato chlorotic spot virus in Sweet Basil (Ocimum basilicum) and Purslane (Portulaca oleracea) in FloridaRichard N. Raid, Joel R. Allingham, Joseph E. Funderburk, Thomas Skarlinsky, Samuel F. Hutton, William W. Turechek, and Scott Adkins6 July 2017 | Plant Health Progress, Vol. 18, No. 2Tospoviruses occurring in and outside JapanJapanese Journal of Phytopathology, Vol. 82, No. 3