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Molecular detection of Mycobacterium bovis in cattle herds of the state of Pernambuco, Brazil

2016; BioMed Central; Volume: 12; Issue: 1 Linguagem: Inglês

10.1186/s12917-016-0656-1

1746-6148

Renata Duarte da Silva Cezar, Norma Lucena‐Silva, Antônio Fernando Barbosa Batista Filho, Jonas de Melo Borges, Pollyane Raysa Fernandes de Oliveira, Érica Chaves Lúcio, Maíra Arruda-Lima, Vânia Lúcia de Assis Santana, José Wilton Pinheiro,

Microbial infections and disease research

The present study aimed to direct detect Mycobacterium bovis in milk (n = 401) and blood (n = 401) samples collected from 401 dairy cows of 20 properties located in the state of Pernambuco, Brazil, by real-time quantitative PCR (qPCR) targeting the region of difference 4 (RD4). Risk factors possibly associated with bovine tuberculosis (BTB) were also evaluated.Of the 802 samples analyzed, one milk (0.25%) and eight blood (2%) samples were positive for M. bovis in the qPCR and their identities were confirmed by sequencing. Animals positive for M. bovis were found in six (30%) of the 20 properties visited. None of the risk factors evaluated were statistically associated with BTB.M. bovis DNA was detected in one milk sample what may pose a risk to public health because raw milk is commonly consumed in Brazil.