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Efecto de enzimas fibrolíticas glucosiladas en la digestibilidad in vitro de MS y MO de alfalfa (Medicago sativa) y ballico (Lolium perenne)

2001; Universidad de San Carlos de Guatemala; Volume: 11; Issue: 6 Linguagem: Inglês

2224-5545

Juan Manuel Pinos‐Rodríguez, Sergio S. González Muñoz, Germán Mendoza Martínez, Ricardo Bárcena Gama, Mario Cobas Peralta,

Ruminant Nutrition and Digestive Physiology

This study was made to measure in vitro effects of exogenous fibrolytic enzymes, protected by glicolisation on in vitro degradation in dry matter (DM) and organic matter (OM) of alfalfa (Medicago sativa) and rye grass (Lolium perenne). First phase of Tilley and Terry technique at 3, 6, 12, 24, 48 and 72 h of incubation was carried out using McDougall saliva and McDougall saliva + rumen fluid. Net disappearance of fibrolytic enzyme substrates was measured in order to compare the effects of the fibrolytic enzymes with or without rumen microorganisms. Fibrolytic enzymes combined with rumen fluid increased (P D.05) with or without rumen fluid. However, at 24 and 48 h net disappearance of alfalfa DM and OM caused by enzymes was lower (P<0.05) than that of rumen fluid, whereas net disappearance of rye grass DM at 48 and 72 h, and of OM at 48 h, was higher (P<0.05) with rumen fluid than without it. Exogenous fibrolytic enzymes improve in vitro digestibility of DM and OM of alfalfa (Medicago sativa) and rye grass (Lolium perenne), even when ruminal microorganisms are present.