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Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production

2014; Springer Science+Business Media; Linguagem: Inglês

10.1007/978-1-4939-2205-5_2

1940-6029

Agustín Correa, Pablo Oppezzo,

Microbial Metabolic Engineering and Bioproduction

Despite the importance of recombinant protein production in academy and industrial fields, many issues concerning the expression of soluble and homogeneous product are still unsolved. Although several strategies were developed to overcome these obstacles, at present there is no magic bullet that can be applied for all cases. Indeed, several key expression parameters need to be evaluated for each protein. Among the different hosts for protein expression, Escherichia coli is by far the most widely used. In this chapter, we review many of the different tools employed to circumvent protein insolubility problems.