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Molecular phylogenetic analyses of tissue coccidia (sarcocystidae; apicomplexa) based on nuclear 18s RDNA and mitochondrial COI sequences confirms the paraphyly of the genus Hammondia

2016; Cambridge University Press; Volume: 2; Linguagem: Inglês

10.1017/pao.2015.7

2055-7094

Mosun E. Ogedengbe, Joseph D. Ogedengbe, Julia Whale, KRISTIN ELLIOT, Marco A. Juárez-Estrada, John R. Barta,

Coccidia and coccidiosis research

SUMMARY Partial mitochondrial cytochrome c oxidase subunit I (mt COI) sequences were generated from: Toxoplasma gondii (strains CTG, GTI, MAS, ME49, PTG, TgCatBr5, TgCat, Br64, TgCgCal, TgToucan); Neospora caninum (Strain NC1); Hammondia hammondi (Strain H.H–20); H. heydorni; H . cf. triffittae; Cystoisospora felis; C. suis; C. canis; C. rivolta; C . cf. ohioensis; Caryospora bigenetica; Sarcocystis rileyi ; and S. neurona . Nuclear 18S rDNA sequences were generated for H. heydorni, H. hammondi, C. suis, C. canis, C. felis, C. rivolta, C . cf. ohioensis, S. neurona , and S. rileyi . Aligned, concatenated 18S rDNA and COI sequences were Bayesian analysed using partitioned nucleotide substitution models [HKY + I + G for 18S; GTR + I + G codon (code = metmt) for COI]. Phylogenetic hypotheses supported a monophyletic Sarcocystidae and its subfamilie with two major clades within the Toxoplasmatinae: (1) a monophyletic clade of Cystoisospora spp. with Nephroisospora eptesici ; and (2) a clade of Toxoplasma, Neospora and Hammondia . Within the latter, Hammondia was shown to be paraphyletic; H. heydorni and H. triffittae were monophyletic with N. caninum [canine definitive hosts (DHs)], whereas H. hammondi was monophyletic with T. gondii (feline DHs). A new genus is erected to resolve the paraphyly of the genus Hammondia confirmed using mt COI and combined 18S/COI sequence datasets.