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Human malignant mesothelioma is recapitulated in immunocompetent BALB/c mice injected with murine AB cells

2016; Nature Portfolio; Volume: 6; Issue: 1 Linguagem: Inglês

10.1038/srep22850

2045-2322

Rosanna Mezzapelle, Eltjona Rrapaj, Elena Gatti, Chiara Ceriotti, Francesco De Marchis, Alessandro Preti, Antonello E. Spinelli, Laura Perani, Massimo Venturini, Silvia Valtorta, Rosa Maria Moresco, Lorenza Pecciarini, Claudio Doglioni, Michela Frenquelli, Luca Crippa, Camilla Recordati, Eugenio Scanziani, Hilda de Vries, Anton Berns, Roberta Frapolli, Renzo Boldorini, Maurizio D’Incalci, Marco E. Bianchi, Massimo P. Crippa,

Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis

Abstract Malignant Mesothelioma is a highly aggressive cancer, which is difficult to diagnose and treat. Here we describe the molecular, cellular and morphological characterization of a syngeneic system consisting of murine AB1, AB12 and AB22 mesothelioma cells injected in immunocompetent BALB/c mice, which allows the study of the interplay of tumor cells with the immune system. Murine mesothelioma cells, like human ones, respond to exogenous High Mobility Group Box 1 protein, a Damage-Associated Molecular Pattern that acts as a chemoattractant for leukocytes and as a proinflammatory mediator. The tumors derived from AB cells are morphologically and histologically similar to human MM tumors, and respond to treatments used for MM patients. Our system largely recapitulates human mesothelioma, and we advocate its use for the study of MM development and treatment.