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Parasite Lactate Dehydrogenase as an Assay for Plasmodium falciparum Drug Sensitivity

1993; American Society of Tropical Medicine and Hygiene; Volume: 48; Issue: 6 Linguagem: Inglês

10.4269/ajtmh.1993.48.739

1476-1645

Michael T. Makler, Judith Ries, Jeffery A. Williams, J E Bancroft, Robert C. Piper, Bruce L. Gibbins, David J. Hinrichs,

Malaria Research and Control

This report compares the use of the lactate dehydrogenase (pLDH) assay with 3H-hypoxanthine incorporation and Giemsa microscopy for the evaluation of anti-malaria drug inhibition of the growth of P. falciparum in vitro. The inhibition profiles and IC50 determinations of the pLDH assay were directly comparable to those determined by the radioactive uptake and microscopic methods. Furthermore, the pLDH culture sensitivity assay is reproducible, easily interpreted, rapid and inexpensive to perform, suggesting field applicability.