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Rapid determination of the synthetic activity of lipases/esterases via transesterification and esterification zymography

2016; Elsevier BV; Volume: 177; Linguagem: Inglês

10.1016/j.fuel.2016.02.079

1873-7153

Jaqueline G. Duarte, Kassia Leone-Ignacio, José André C. da Silva, Roberto Fernandéz‐Lafuente, Denise Maria Guimarães Freire,

Biodiesel Production and Applications

A new simple and extremely versatile zymography method based on transesterification and esterification reactions was developed in this work. The method consists in building a transesterification or esterification reaction medium according to the goal of the research. Since commercial enzymes and crude extracts commonly consist in pools of proteins, this methodology provides a means to determine which protein are responsible for the enzymatic activity desired. The protocol may be potentially used as a high-throughput screening of lipases or esterases that catalyzes the synthesis reaction for biodiesel and biolubricants production.