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Distribution of the H1 Histone Subfractions in Syrian Hamster Chromatin Fractions

1984; De Gruyter; Volume: 39; Issue: 9-10 Linguagem: Inglês

10.1515/znc-1984-9-1015

1865-7125

Hanna Modrzejewska, Grażyna Gałązka, Janusz Szemraj, H Panusz,

Genomics and Chromatin Dynamics

Abstract Chromatin from two Syrian hamster tissues: the Kirkman-Robbins hepatoma and the liver, has been separated into soluble (S) and insoluble (P) fractions. Both fractions contain the complete set of five main histones but differ in respect of H1 subfractions. The hepatoma chromatin is known to contain an unusual H1 subfraction, H1 slow [12, 13], probably identical with a similar subfration present in hamster testes. The content of H1 slow in total H1 histone has been estimated for total, S and P chromatin from hamster hepatoma. The values 20.9 ± 7.2, 13.8 ± 1.8 and 26.8 ± 4.2%, respectively, were obtained.