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Dissection of Individual Prostate Lobes in Mouse Models of Prostate Cancer to Obtain High Quality RNA

2016; Wiley; Volume: 232; Issue: 1 Linguagem: Inglês

10.1002/jcp.25384

1097-4652

Areg Zingiryan, Nicholas H. Farina, Kristiaan H. Finstad, Janet L. Stein, Jane B. Lian, Gary S. Stein,

RNA Research and Splicing

Journal of Cellular PhysiologyVolume 232, Issue 1 p. 14-18 From The Bench Dissection of Individual Prostate Lobes in Mouse Models of Prostate Cancer to Obtain High Quality RNA Correction(s) for this article Cover Image, Volume 232, Number 1, January 2017 (page i) Areg Zingiryan, Nicholas H. Farina, Kristiaan H. Finstad, Janet L. Stein, Jane B. Lian, Gary S. Stein, Volume 232Issue 11Journal of Cellular Physiology pages: 3194-3194 First Published online: July 25, 2017 Areg Zingiryan, Areg Zingiryan Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VermontSearch for more papers by this authorNicholas H. Farina, Corresponding Author Nicholas H. Farina nicholas.farina@med.uvm.edu Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, Vermont Correspondence to: Nicholas H. Farina, University of Vermont, 89 Beaumont Avenue, E209 Given Building, Burlington, VT 05405. E-mail: nicholas.farina@med.uvm.eduSearch for more papers by this authorKristiaan H. Finstad, Kristiaan H. Finstad Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VermontSearch for more papers by this authorJanet L. Stein, Janet L. Stein Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VermontSearch for more papers by this authorJane B. Lian, Jane B. Lian Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VermontSearch for more papers by this authorGary S. Stein, Gary S. Stein Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VermontSearch for more papers by this author Areg Zingiryan, Areg Zingiryan Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VermontSearch for more papers by this authorNicholas H. Farina, Corresponding Author Nicholas H. Farina nicholas.farina@med.uvm.edu Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, Vermont Correspondence to: Nicholas H. Farina, University of Vermont, 89 Beaumont Avenue, E209 Given Building, Burlington, VT 05405. E-mail: nicholas.farina@med.uvm.eduSearch for more papers by this authorKristiaan H. Finstad, Kristiaan H. Finstad Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VermontSearch for more papers by this authorJanet L. Stein, Janet L. Stein Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VermontSearch for more papers by this authorJane B. Lian, Jane B. Lian Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VermontSearch for more papers by this authorGary S. Stein, Gary S. Stein Department of Biochemistry, University of Vermont Cancer Center, University of Vermont College of Medicine, Burlington, VermontSearch for more papers by this author First published: 18 March 2016 https://doi.org/10.1002/jcp.25384Citations: 8 Conflicts of interest: The authors have no conflicts of interest to declare. Areg Zingiryan and Nicholas H. Farina contributed equally to this work. Present address of Dr. Zingiryan is Department of Medicine, Internal Medicine, Lewis Katz School of Medicine, Temple University, Philadelphia, Pennsylvania. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Genetically engineered mouse models of prostate cancer allow for study of disease progression from localized tumor formation through distal metastasis. The anatomy of the mouse prostate differs dramatically from the human prostate, being composed of four lobe pairs (anterior, dorsal, lateral, and ventral), making the identification and dissection technically challenging. Although the entire murine prostate and surrounding tissue, including urethra, bladder, seminal vesicles, and associated adipose tissue, can be quickly dissected for en bloc analysis, it is necessary to isolate individual prostate lobes for gene expression studies elucidating the molecular mechanisms of prostate cancer. The procedure as described here includes full color images, allowing the researcher to appreciate the unique prostate morphology and tissue manipulation required to harvest individual prostate lobes. Along with removing all extraneous tissue, the procedure allows for direct comparison of the different prostate lobes by established downstream techniques. Importantly, high quality RNA required for next-generation gene expression analysis can only consistently be obtained from ventral and lateral lobes. Finally, preclinical studies using prostate targeted therapies can be monitored specifically in individual prostate lobes for histological and gene expression studies. J. Cell. Physiol. 232: 14–18, 2017. © 2016 Wiley Periodicals, Inc. Citing Literature Supporting Information Additional supporting information may be found in the online version of this article at the publisher's web-site. Filename Description jcp25384-sup-0001-SuppFig-S1.eps81.7 MB Figure S1 jcp25384-sup-0002-SuppData-S2.docx174.2 KB Supporting Information. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume232, Issue1January 2017Pages 14-18 RelatedInformation