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Large-Scale Gene Disruption Using the UAU1 Cassette

2009; Springer Science+Business Media; Linguagem: Inglês

10.1007/978-1-60327-151-6_17

1940-6029

Clarissa J. Nobile, Aaron P. Mitchell,

Nematode management and characterization studies

Candida albicans is a major fungal systemic pathogen in humans. Genetic manipulation of C. albicans is unwieldy. We report here a strategy that is useful and successful for large-scale genetic manipulation of C. albicans genes of interest: use of the UAU1 cassette on a Tn7 transposon. Streamlined yet admittedly flawed disruption techniques, such as the one described here, may prove vital to uncovering the genetic basis of fungal virulence.