Produção Nacional
Revisado por pares
Microwave assisted direct saponification for the simultaneous determination of cholesterol and cholesterol oxides in shrimp
2016; Elsevier BV; Volume: 169; Linguagem: Inglês
10.1016/j.jsbmb.2016.03.027
ISSN1879-1220
AutoresHugo Antonio Lima de Souza, Lilian Regina Barros Mariutti, Neura Bragagnolo,
Tópico(s)Cholesterol and Lipid Metabolism
ResumoA novel microwave-assisted direct saponification method for the simultaneous determination of cholesterol and cholesterol oxides in shrimp was developed and validated. Optimal saponification conditions, determined by means of an experimental design, were achieved using 500 mg of sample and 20 mL of 1 mol/L KOH ethanol solution for 16 min at 45 °C at maximum power at 200 W and magnetic stirring at 120 rpm. Higher extraction of cholesterol oxides in a reduced saponification time (∼75 times) was achieved in comparison with the direct cold saponification method. The new method showed low detection (≤0.57 μg/mL) and quantification (≤1.73 μg/mL) limits, good repeatability (≤10.50% intraday and ≤8.56% interday) and low artifact formation (evaluated by using a deuterated cholesterol-D6 standard). Raw, salted and dried-salted shrimps were successfully analyzed by the validated method. The content of cholesterol oxides increased after salting and decreased after drying.
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