CARACTERIZACIÓN MOLECULAR DE VARIEDADES DE VID (Vitis vinifera L.) DE CALIDAD ENOLÓGICA POR MARCADORES MICROSATÉLITES MOLECULAR CHARACTERIZATION OF GRAPE VARIETIES (Vitis vinifera L.) OF ENOLOGICAL VALUE USING MICROSATELLITES MARKERS
2006; Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo; Linguagem: Inglês
ISSN
1853-8665
AutoresLiliana Martínez, Pablo F. Cavagnaro, Ricardo W. Masuelli,
Tópico(s)Wine Industry and Tourism
ResumoSSR markers characterized seven grapevine varieties used for high quality wines in Argentina. Six of the 8 primers used gave reproducible band profiles. The number of alleles per locus varied from 5 to 10, with a total of 42 and 1 to 7 unique alleles. Three to 7 SSR genotypes per locus were found, with a total of 28. Tempranillo and Chenin showed 6 and 5 unique alleles, respectively, taking in account all the primers assessed. The observed heterozygosity varied between 42.9 and 100 %, while the expected heterozygosity ranged from 64.3 to 87.8 %. VrZAG79 was the most informative locus (PIC 84.9 % and ne 8.17), while VrZAG62 was the least informative. Combining the use of 6 primers, the accumulative probability of missidentifying two random different genotypes as the same, was very low: 7.68 x 10-05. The UPGMA cluster analysis separated the French varieties (Merlot, Pinot Noir, Cabernet Sauvignon), and Syrah from Tempranillo and Bonarda. This technique can provide a service to grapevine nurseries and farmers interested in certifying the genetic identity of the materials commercialized by them.
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