Raman spectroscopy as a tool for label-free lymphocyte cell line discrimination
2016; Royal Society of Chemistry; Volume: 141; Issue: 12 Linguagem: Inglês
10.1039/c6an00181e
ISSN1364-5528
AutoresAlison J. Hobro, Yutaro Kumagai, Shizuo Akira, Nicholas I. Smith,
Tópico(s)Digital Imaging for Blood Diseases
ResumoUnactivated lymphocytes are morphologically identical and biochemically relatively similar, making them difficult to distinguish from one another with conventional light microscopy. Here, we use Raman spectroscopy to provide biochemical information on the composition of different lymphocyte cell lines. As could be expected, the biochemical differences measured with Raman spectroscopy between lymphocyte cell lines are small, but in combination with partial least squares discriminant analysis it is possible not only to distinguish between T- and B-cells, but also between individual T-cell and B-cell lines.
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