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CHARACTERIZATION OF METAL HOMEOSTASIS RELATED RICE GENE ORTHOLOGS INNUTRI- RICH MINOR MILLETS

2015; Volume: 2015; Linguagem: Inglês

2231-4490

G J Patel, Monika Dubey, Girish Chandel,

Plant Stress Responses and Tolerance

Minor millets are nutritious food crops which bear potentials to serve as future food to combat the deep rooted malnutrition and nutritional insecurity prevailing in the developing world. The presented study focuses on characterizing different minor millet genotypes for grain micronutrient content along with the expression analysis of related genes. The study revealed higher level of iron and zinc content in millet grains as compared to major cereal crops. Fe content ranged from 25.65 μg/g in Kodo millet to 42.13 μg/g in Barnyard millet. In regard to grain zinc content, it ranged from 20.66 μg/g in Kodo millet to 54.34 μg/g in Barnyard millet. Expression analysis of sixteen metal homeostasis related rice gene orthologs depicted variable gene expression levels in seven genotypes of minor millets in flag leaf tissues at mid grain filling stage. VIT1 gene showed expression in all minor millets genotype with high level of expression in Sawa local, Melaghat-1, Melaghat-2, RLM-37, and TNAU-86. OsYSL2 and OsYSL18 genes depicted exceptionally high level of expression in flag leaf tissue in Kodo millet genotype TNAU-86. Similarly, NRAMP5 gene transcript showed extreme expression level in TNAU-86 followed by Melaghat-2 and RLM-37. Attempt to establish association between gene expression and grain Fe and Zn levels showed co linearity between these two parameters. Expression of three genes (YSL 2, NAS 1, NAS 3) was found to be correlated to high grain Fe contents, one gene (NAAT) to high grain Zn contents whereas expression of ten genes (IRT 1, NAS 2, ZIP 7, ZIP 5, ZIP 1, FER 1, FER 2, NAC 5, NRAMP 5, NRAMP 7) was found to be correlated to both high Fe and Zn contents.