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Direct and cost-efficient hyperpolarization of long-lived nuclear spin states on universal 15 N 2 -diazirine molecular tags

2016; American Association for the Advancement of Science; Volume: 2; Issue: 3 Linguagem: Inglês

10.1126/sciadv.1501438

2375-2548

Thomas Theis, Gerardo X. Ortiz, Angus W. J. Logan, Kevin Claytor, Yesu Feng, William Huhn, Volker Blüm, Steven J. Malcolmson, Eduard Y. Chekmenev, Qiu Wang, Warren S. Warren,

Electron Spin Resonance Studies

Conventional magnetic resonance (MR) faces serious sensitivity limitations which can be overcome by hyperpolarization methods, but the most common method (dynamic nuclear polarization) is complex and expensive, and applications are limited by short spin lifetimes (typically seconds) of biologically relevant molecules. We use a recently developed method, SABRE-SHEATH, to directly hyperpolarize (15)N2 magnetization and long-lived (15)N2 singlet spin order, with signal decay time constants of 5.8 and 23 minutes, respectively. We find >10,000-fold enhancements generating detectable nuclear MR signals that last for over an hour. (15)N2-diazirines represent a class of particularly promising and versatile molecular tags, and can be incorporated into a wide range of biomolecules without significantly altering molecular function.