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Use of the Fluidigm C1 platform for RNA sequencing of single mouse pancreatic islet cells

2016; National Academy of Sciences; Volume: 113; Issue: 12 Linguagem: Inglês

10.1073/pnas.1602306113

1091-6490

Yurong Xin, Jinrang Kim, Min Ni, Yi Wei, Haruka Okamoto, Joseph Lee, Christina Adler, Katie Cavino, Andrew Murphy, George D. Yancopoulos, Hsin Chieh Lin, Jesper Gromada,

Pancreatic and Hepatic Oncology Research

Significance Pancreatic islets are complex structures composed of four cell types whose primary function is to maintain glucose homeostasis. Owing to the scarcity and heterogeneity of the islet cell types, little is known about their individual gene expression profiles. Here we used the Fluidigm C1 platform to obtain high-quality gene expression profiles of each islet cell type from mice. We identified cell-type–specific transcription factors and pathways providing previously unrecognized insights into genes characterizing islet cells. Unexpectedly, our data uncover technical limitations with the C1 Fluidigm cell capture process, which should be considered when analyzing single-cell transcriptomics data.