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RESISTÊNCIA A METAIS PESADOS, ANTIMICROBIANOS E FORMAÇÃO DE BIOFILME EM CEPAS DE ESCHERICHIA COLI ISOLADAS DE PRAIAS DE SÃO LUIS - MARANHÃO

2014; UNIVERSIDADE FEDERAL DE GOIÁS; Volume: 43; Issue: 3 Linguagem: Inglês

10.5216/rpt.v43i3.32213

ISSN

1980-8178

Autores

André Luiz Raposo Barros, Márcio Fernandes Alves Leite, Ronildson Lima Luz, Nathalia Cunha Almeida Pinheiro, Monique Santos do Carmo, Flávio Henrique Reis Moraes, Patrícia de Maria Silva Figueirêdo,

Tópico(s)

Fecal contamination and water quality

Resumo

The city of São Luís located in the State of Maranhão has inefficient treatment of their effluent, released into the rivers or directly on the beaches. This study evaluated the resistance of 39 strains of E. coli to Cu, Ni, Cd e Hg, their susceptibility to antibiotics and ability of biofilm production. Samples were collected from the beaches of Ponta D'Areia, São Marcos, Calhau and Olho d’Água. The antibiotic susceptibility was performed by disk diffusion method with the following antibiotics: Aztreonam (ATM), Imipenem (IMP), Cephalothin (KF); Ampicillin / Sulbactam (ASB), Gentamicin (CN), Ampicillin (AMP); Cefuroxime Sodium (CXM), Amoxicillin (AML); Piperacillin Tazobactam (TZP), Trimethoprim-sulfamethoxazole (SXT), Amoxicillin + clavulanic acid (AMC). The detection of biofilms production were performed in Congo Red Agar (CRA) and spectrophotometry (ESP). The results showed 2 strains Hg tolerant (1 mM), 9 strains resistant to Cu and Cd (4 mM) and 12 strains resitente to Ni (4 mM). The antibiotic susceptibility tests showed resistance to ATM (15% of samples), for KF (95%), BSA (85%), CN (5%), AMP (87%), CXM (13%), TZP (5%) SXT (74%) and AMC (18%). For the synthesis of biofilm in CRA 20% showed strong production, 36% low and 44% did not produce. By spectrophotometry only 1 (2.5%) proved to be moderate biofilm producer, 5 (12.5%) were classified as non-producers and other 34 (85%) weak produce. The presence of bacteria with antimicrobial and heavy metal resistance profile, and production of biofilms represents a public health risk and direct changes in ecological communities of marine microorganisms.

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